Prostaglandin F2alpha ELISA Kit

ELISA Kit

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Prostaglandin F2alpha

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology Prostaglandin F2alpha ELISA Kit (OKEH02618) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Prostaglandin F2alpha antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Prostaglandin F2alpha and incubated. The Prostaglandin F2alpha found in the sample or standards competes with the biotinylated Prostaglandin F2alpha for limited binding sites on the immobilized anti-Prostaglandin F2alpha antibody. Excess unbound biotinylated Prostaglandin F2alpha and sample or standard Prostaglandin F2alpha is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Prostaglandin F2alpha captured in the well and inversely proportional to the amount of Prostaglandin F2alpha which was contained in the sample or standard.

Mean Intra-assay CV%: < 6.1% (n = 20)
Mean Inter-assay CV%: < 8.5% (n = 20)