FTO ELISA Kit (Human)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

58 kDa

FTO alpha-ketoglutarate dependent dioxygenase

Store as indicated in product manual.

NM_001080432.2

Q9C0B1

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology FTO ELISA Kit (Human) (OKEH03328) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FTO has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for FTO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FTO captured in well.

Mean Intra-assay CV%: <= 7.2% (n = 20)
Mean Inter-assay CV%: <= 8.5% (n = 20)