CTCF ELISA Kit (Mouse)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

84 kDa

CCCTC-binding factor

Store as indicated in product manual.

NM_181322.3

Q61164

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology Ctcf ELISA Kit (Mouse) (OKEH03508) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Mouse Ctcf antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ctcf and incubated. The Ctcf found in the sample or standards competes with the biotinylated Ctcf for limited binding sites on the immobilized anti-Mouse Ctcf antibody. Excess unbound biotinylated Ctcf and sample or standard Ctcf is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ctcf captured in the well and inversely proportional to the amount of Ctcf which was contained in the sample or standard.

Mean Intra-assay CV%: <= 7.2% (n = 20)
Mean Inter-assay CV%: <= 9.4% (n = 20)