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PAF ELISA Kit (Mouse)

PAF ELISA Kit (Mouse)

Item no.	OKEH03565
Manufacturer	AVIVA Systems Biology
Amount	96 tests
Category	Oncology Neuroscience Neural Lineage Markers By Cell Type Blastoma Carcinoma Cell Biology Cell Status ELISA Proliferation ELISA & Immunoassays ELISA Kits Immunoassay By Organ Bladder Cancer Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Mouse (Murine, Mus musculus)
Sensitivity	40 pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	2810417H13Rik;AA409629;HCV NS5A-transactivated protein 9 homolog;mKIAA0101;Ns5apt9;Ns5atp9;p15(P;p15(PAF);p15PAF;Paf;PAF15;PCNA-associated factor;PCNA-associated factor of 15 kDa;PCNA-clamp-associated factor.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Molecular Weight	12 kDa
Gene symbol	Pclaf
Gene Fullname	PCNA clamp associated factor
Protein size	110
Reconstitution and storage	Store as indicated in product manual.
Description of target	PCNA-associated factor
Nucleotide accession_num	NM_026515.2
Protein accession_num	NP_080791.2
Protein name	Q9CQX4
Manufacturer - Specificity	Natural and recombinant Mouse PCNA-associated factor
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit duration	~ 3 Hours
Kit principle	Aviva Systems Biology PAF ELISA Kit (Mouse) (OKEH03565) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Paf has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Paf is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Paf captured in well.
Kit recovery	Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 88%
Kit reproducibility	Mean Intra-assay CV%: <= 6.4% (n = 20) Mean Inter-assay CV%: <= 8.3% (n = 20)
Sample Type	Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKEH03565.pdf

OKEH03565-datasheet.pdf

OKEH03565-safety-datasheet.pdf