PPME1 ELISA Kit (Rat)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

42 kDa

protein phosphatase methylesterase 1

Store as indicated in product manual.

NM_001191838.1

Q4FZT2

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology PPME1 ELISA Kit (Rat) (OKEH03629) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human Ppme1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Ppme1 and incubated. The Ppme1 found in the sample or standards competes with the biotinylated Ppme1 for limited binding sites on the immobilized anti-human Ppme1 antibody. Excess unbound biotinylated Ppme1 and sample or standard Ppme1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ppme1 captured in the well and inversely proportional to the amount of Ppme1 which was contained in the sample or standard.