HSD17B3 ELISA Kit (Rat)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

34 kDa

hydroxysteroid (17-beta) dehydrogenase 3

Store as indicated in product manual.

NM_054007.1

O54939

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology HSD17B3 ELISA Kit (Rat) (OKEH03714) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Hsd17b3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Hsd17b3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Hsd17b3 captured in well.