VIP ELISA Kit (Chicken)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

200 kDa

vasoactive intestinal peptide

Store as indicated in product manual.

NM_001177309.1

P48143

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology VIP ELISA Kit (Chicken) (OKEH03960) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human VIP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated VIP and incubated. The VIP found in the sample or standards competes with the biotinylated VIP for limited binding sites on the immobilized anti-human VIP antibody. Excess unbound biotinylated VIP and sample or standard VIP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated VIP captured in the well and inversely proportional to the amount of VIP which was contained in the sample or standard.