HIF1AN ELISA Kit (Human)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

40 kDa

hypoxia inducible factor 1 subunit alpha inhibitor

Store as indicated in product manual.

NM_017902.2

Q9NWT6

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology HIF1AN ELISA Kit (Human) (OKEH04329) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HIF1AN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HIF1AN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HIF1AN captured in the well.

Mean Intra-assay CV%: <= 5.1% (n = 20)
Mean Inter-assay CV%: <= 7.9% (n = 20)