Comparison

SOD1 ELISA Kit (Dog)

Item no. OKEH04617
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Applications ELISA
Specific against Dog (Canine, Canis lupus familiaris)
Sensitivity 0.086 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Cu/Zn superoxide dismutase;superoxide dismutase [Cu-Zn];superoxide dismutase 1 soluble;superoxide dismutase 1,soluble (amyotrophic lateral sclerosis 1 (adult)).
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Molecular Weight
16 KDa
Gene symbol
SOD1
Gene Fullname
superoxide dismutase 1
Protein size
153
Reconstitution and storage
Store as indicated in product manual.
Description of target
Superoxide dismutase [Cu-Zn]
Nucleotide accession_num
NM_001003035.1
Protein accession_num
NP_001003035.1
Protein name
Q8WNN6
Manufacturer - Specificity
Natural and recombinant Dog Superoxide dismutase [Cu-Zn]
Assay info
Assay Methodology: Quantitative Competitive ELISA
Kit duration
~3 Hours
Kit principle
Aviva Systems Biology SOD1 ELISA Kit (Dog) (OKEH04617) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Dog SOD1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SOD1 and incubated. The found in the sample or standards competes with the biotinylated SOD1 for limited binding sites on the immobilized anti-Dog SOD1 antibody. Excess unbound biotinylated SOD1 and sample or standard SOD1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SOD1 captured in the well and inversely proportional to the amount of SOD1 which was contained in the sample or standard.
Kit reproducibility
Mean Intra-Assay CV: < 10%
Mean Inter-Assay CV: < 12%
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
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