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INPP5D ELISA Kit (Rat)

INPP5D ELISA Kit (Rat)

Item no.	OKEH05967
Manufacturer	AVIVA Systems Biology
Amount	96 tests
Category	Oncology Neuroscience Neural Signaling By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cell Culture Cytokines Cell Status Adenocarcinoma Mesothelioma ELISA Autophagy Cardiovascular Research Proliferation Myocardial Infarction ELISA & Immunoassays ELISA Kits Immunoassay
Type	Elisa-Kit
Specific against	Rat (Rattus norvegicus)
Sensitivity	0.88 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1,SH2 domain-containing inositol-5'-phosphatase 1,SH2 domain-containing inositol phosphatase 1,Ship,Ship1,SHIP-1
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	INPP5D
Gene Fullname	Inositol polyphosphate-5-phosphatase D
Protein size	1190
Reconstitution and storage	Store as indicated in product manual.
Description of target	Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3, 4, 5-trisphosphate (PtdIns(3, 4, 5)P3) to produce PtdIns(3, 4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways. Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression. May also hydrolyze PtdIns(1, 3, 4, 5)P4, and could thus affect the levels of the higher inositol polyphosphates like InsP6.
Nucleotide accession_num	NM_019311.1
Protein accession_num	NP_062184.1
Protein name	Phosphatidylinositol 3, 4, 5-trisphosphate 5-phosphatase 1
Manufacturer - Specificity	Natural and recombinant Rat Phosphatidylinositol 3, 4, 5-trisphosphate 5-phosphatase 1
Assay info	Assay Methodology: Quantitative Competitive ELISA
Kit duration	~3 Hours
Kit principle	Aviva Systems Biology Inpp5d ELISA Kit (Rat) (OKEH05967) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Inpp5d has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Inpp5d is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Inpp5d captured in well.
Kit recovery	Mean recovery when spiking into Serum and Plasma = 89%
Kit reproducibility	Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision. Mean Intra-Assay Precision - <5.1%, n = 20 Mean Inter-Assay Precision - <8.9%, n = 20
Sample Type	Serum, plasma and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 96 tests

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Delivery expected until 10/30/2025

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