EPHA3 ELISA Kit (Rat)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

Eph receptor A3

Store as indicated in product manual.

NM_031564.1

O08680

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology Epha3 ELISA Kit (Rat) (OKEH06230) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Epha3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Epha3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Epha3 captured in the well.

Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <4.7%, n = 20
Mean Inter-Assay Precision - <9.2%, n = 20