ADRA1A ELISA Kit (Bovine)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

ADRA1A

Store as indicated in product manual.

NM_174498

P18130

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology Adra1a ELISA Kit (Bovine) (OKEH06638) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Adra1a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Adra1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Adra1a captured in the well.

Serum, Plasma, Tissue Homogenates