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PLA2G2A ELISA Kit (Bovine)

PLA2G2A ELISA Kit (Bovine)

Item no.	OKEH06688
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Metabolism Cholesterol & Lipid Metabolism ELISA Enzymes Fatty Acid Catabolism ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Cow (Bos taurus), Cattle (Bovine)
Sensitivity	0.092 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	GIIC sPLA2;group IIA phospholipase A2;phosphatidylcholine 2-acylhydrolase 2A;phospholipase A2,membrane associated.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	PLA2G2A
Gene Fullname	phospholipase A2, group IIA (platelets, synovial fluid)
Reconstitution and storage	Store as indicated in product manual.
Description of target	Phospholipase A2, membrane associated
Nucleotide accession_num	NM_001025324
Protein accession_num	NP_001020495
Protein name	Q56JZ2
Manufacturer - Specificity	Natural and recombinant Bovine Phospholipase A2, membrane associated
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit duration	~3 Hours
Kit principle	Aviva Systems Biology Pla2g2a ELISA Kit (Bovine) (OKEH06688) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Pla2g2a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Pla2g2a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Pla2g2a captured in the well.
Kit reproducibility	Intra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates. Intra-Assay CV <10% Inter-Assay CV <12%
Sample Type	Serum, Plasma, Tissue Homogenates

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKEH06688.pdf

OKEH06688-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 10/30/2025

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