HSD11B2 ELISA Kit (Rat)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

hydroxysteroid 11-beta dehydrogenase 2

Corticosteroid 11-beta-dehydrogenase isozyme 2

NP_058777.1

Natural and recombinant Rat Corticosteroid 11-beta-dehydrogenase isozyme 2

Aviva Systems Biology HSD11B2 ELISA Kit (Rat) (OKEH07747) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HSD11B2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HSD11B2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HSD11B2 captured in well.

Mean Intra-assay CV%: <=9.4% (n = 20)
Mean Inter-assay CV%: <=11.9% (n = 20)