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AKT1S1 ELISA Kit (Human)

AKT1S1 ELISA Kit (Human)

Item no.	OKEH07996
Manufacturer	AVIVA Systems Biology
Amount	96 tests
Category	Oncology Neuroscience Aging & Neurological Disorders By Cell Type Blastoma Carcinoma Hematology Lymphoma Myeloma Melanoma Sarcoma ELISA ELISA & Immunoassays ELISA Kits Immunoassay Immunostaining
Type	Elisa-Kit
Applications	ELISA
Specific against	Human (Homo sapiens)
Sensitivity	0.057ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	40 kDa proline-rich AKT substrate;AKT1 substrate 1 (proline rich);Lobe;PRAS40;proline-rich AKT1 substrate 1.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Applications	Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category	Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits
Shipping Temperature	Wet Ice
Gene symbol	AKT1S1
Gene Fullname	AKT1 substrate 1
Reconstitution and storage	Store as indicated in product manual.
Description of target	Proline-rich AKT1 substrate 1
Nucleotide accession_num	NM_001098632.2
Protein accession_num	NP_001092102.1
Protein name	Q96B36
Manufacturer - Specificity	Natural and recombinant Human Proline-rich AKT1 substrate 1
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit linearity	Sample - 1:2 - 1:4 - 1:8 - 1:16serum(n=5) - 101-111% (n = 20) - 104-114% (n = 20) - 96-106% (n = 20) - 81-81% (n = 20)EDTA plasma(n=5) - 89-99% (n = 20) - 96-108% (n = 20) - 105-113% (n = 20) - 105-115% (n = 20)heparin plasma(n=5) - 91-102% (n = 20) - 105-115% (n = 20) - 106-115% (n = 20) - 106-119% (n = 20)
Kit principle	Aviva Systems Biology AKT1S1 ELISA Kit (Human) (OKEH07996) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AKT1S1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AKT1S1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AKT1S1 captured in well.
Kit recovery	Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 117% (n = 20)
Kit reproducibility	Mean Intra-assay CV%: <=3.3% (n = 20) Mean Inter-assay CV%: <=8.6% (n = 20)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKEH07996.pdf

OKEH07996-datasheet.pdf

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Amount: 96 tests

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Delivery expected until 12/25/2025

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