S100A12 ELISA Kit (Pig)

ELISA Kit

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S100A12

Store as indicated in product manual.

NM_001160272.1

P80310

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology S100A12 ELISA Kit (OKEH08123) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-S100A12 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated S100A12 and incubated. The S100A12 found in the sample or standards competes with the biotinylated S100A12 for limited binding sites on the immobilized anti-S100A12 antibody. Excess unbound biotinylated S100A12 and sample or standard S100A12 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated S100A12 captured in the well and inversely proportional to the amount of S100A12 which was contained in the sample or standard.

Mean Intra-assay CV%: <=7.9% (n = 20)
Mean Inter-assay CV%: <=9.2% (n = 20)