SMURF2 ELISA Kit (Human)

ELISA Kit

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SMURF2

Store as indicated in product manual.

NM_022739.3

Q9HAU4

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology SMURF2 ELISA Kit (OKEH08515) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-SMURF2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SMURF2 and incubated. The SMURF2 found in the sample or standards competes with the biotinylated SMURF2 for limited binding sites on the immobilized anti-SMURF2 antibody. Excess unbound biotinylated SMURF2 and sample or standard SMURF2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SMURF2 captured in the well and inversely proportional to the amount of SMURF2 which was contained in the sample or standard.

Mean Intra-assay CV%: <=6.8% (n = 20)
Mean Inter-assay CV%: <=11.9% (n = 20)