Comparison

BCL-2 ELISA Kit (Human)

Item no. OKEI00123
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Specific against Human (Homo sapiens)
Sensitivity 0.094 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Apoptosis regulator Bcl-2,Bcl-2
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
BCL-2
Reconstitution and storage
Store as indicated in product manual.
Protein name
B-cell leukemia/lymphoma-2-alpha protein
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit duration
~ 3 Hours
Kit principle
Aviva Systems Biology BCL-2 ELISA Kit (Human) (OKEI00123) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCL-2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCL-2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample BCL-2
Kit reproducibility
Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level BCL-2. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level BCL-2 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Sample Type
Serum, Plasma, Biological Fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
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