Comparison

GPR50 ELISA Kit (Human)

Item no. OKEI00276
Manufacturer AVIVA Systems Biology
Amount 96 Wells
Category
Type Elisa-Kit
Specific against Human (Homo sapiens)
Sensitivity 9.375 pg/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias H9,Mel1c
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Gene symbol
GPR50
Reconstitution and storage
Store as indicated in product manual.
Description of target
Does not bind melatonin.
Nucleotide accession_num
NM_004224
Protein accession_num
NP_004215
Protein name
Melatonin-related receptor
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit duration
~ 3 Hours
Kit principle
Aviva Systems Biology GPR50 ELISA Kit (Human) (OKEI00276) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GPR50 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GPR50 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample GPR50
Kit reproducibility
Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level GPR50. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level GPR50 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
Sample Type
Serum, Plasma, Biological Fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 Wells
Available: In stock
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