MIP ELISA Kit (Mouse)

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MIP

Water channel. Channel activity is down-regulated by CALM when cytoplasmic Ca2+ levels are increased. May be responsible for regulating the osmolarity of the lens. Interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core.

NP_032626

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology Mip ELISA Kit (Mouse) (OKEI00356) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Mip has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Mip is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of a luminol substrate which is catalyzed by the HRP to produce light emission. The light emission is read by a luminometer (or photo-multiplier equipped instrument) and the intensity of the emitted light is proportional to the amount of sample Mip

Serum, Plasma, Biological Fluids