LAMTOR1 ELISA Kit (Human)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

late endosomal/lysosomal adaptor, MAPK and MTOR activator 1

As part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. LAMTOR1 is directly responsible for anchoring the Ragulator complex to membranes. Also required for late endosomes/lysosomes biogenesis it may regulate both the recycling of receptors through endosomes and the MAPK signaling pathway through recruitment of some of its components to late endosomes. May be involved in cholesterol homeostasis regulating LDL uptake and cholesterol release from late endosomes/lysosomes. May also play a role in RHOA activation.

NP_060377

Assay Type: Quantitative Sandwich ELISA

Colorimetric, OD 450 nm

Sample Type - 1:2 Dilution - 1:4 Dilution - 1:8 Dilution - 1:16 Dilution
Serum (n=5) - 85-105% - 87-99% - 94-102% - 87-100%
EDTA Plasma (n=5) - 90-95% - 83-98% - 86-100% - 86-97%
Heparin Plasma (n=5) - 80-98% - 81-100% - 91-100% - 82-94%

Sample Type - Range - Mean
Serum (n=5) - 86-103 - 95
EDTA Plasma (n=5) - 85-99 - 90
Heparin Plasma (n=5) - 86-104 - 95

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids