IL2 ELISA Kit (Monkey)

Enzyme-linked Immunosorbent assay-Sandwich

Wet Ice

interleukin 2

Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance. Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5. This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance. Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells.

XP_007997918

Assay Type: Quantitative Sandwich ELISA

Colorimetric, OD 450 nm

Sample Type - 1:2 Dilution - 1:4 Dilution - 1:8 Dilution - 1:16 Dilution
Serum (n=5) - 91-105% - 85-104% - 87-103% - 85-97%
EDTA Plasma (n=5) - 89-99% - 84-101% - 83-100% - 93-101%
Heparin Plasma (n=5) - 80-98% - 81-86% - 81-96% - 80-99%

Sample Type - Range - Mean
Serum (n=5) - 86-100 - 94
EDTA Plasma (n=5) - 88-103 - 95
Heparin Plasma (n=5) - 94-103 - 100

Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids