OREXIN A AND B ELISA Kit (Sheep)

ELISA Kit

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OREXIN A AND B

Orexin A and B

Component: Amount
Orexin a and b Microplate: 96 Wells (12 x 8 Well strips)
Orexin a and b Lyophilized Standard: 2 x 1 ug
100X Orexin a and b Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
25X Wash Buffer: 1 x 20 mL
TMB Substrate: 1 x 10 mL
Stop Solution: 1 x 10 mL

~ 3 Hours

Aviva Systems Biology Orexin a and b ELISA Kit (Sheep) (OKWB00437) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Orexin a and b has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Orexin a and b is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Orexin a and b captured in the well.

Mean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%