Mannose Binding Lectin (MBL) BioAssay™ ELISA Kit (Human)

Kits and Assays / Kits-ELISA (Pattern Recognition Receptors), BioAssay™

4°C/-20°C

Mannose Binding Lectin (MBL) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MBL in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Detection Range:
0.156-10ng/ml

Sensitivity:
0.069ng/ml

Intra-Assay CV:
<10%

Inter-Assay CV:
<12%

Kit Components:
*026668A: Microtiter Strips, 1x48 wells or 1x96 wells, Pre-coated, ready to use
*026668B: Standard, 2x1 vial
026668C: Standard Diluent, 1x10ml or 1x20ml
*026668D: Detection Reagent A (green), 1x60ul or 1x120ul
*026668E: Detection Reagent B (red), 1x60ul or 1x120ul
026668F: Assay Diluent A, 1x6ml or 1x12ml
026668G: Assay Diluent B, 1x6ml or 1x12ml
026668H: TMB Substrate, 1x4.5ml or 1x9ml
026668K: Stop Solution, 1x3ml or 1x6ml
026668L: Wash Buffer, 30X, 1x10ml or 1x20ml

Precaution:
The Stop Solution (026668K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Storage and Stability:
Store *026668A, *026668B, *026668D and *026668E at -20°C. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Procedure Summary:
1. Prepare all reagents, samples and standards.
2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C.
3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C.
4. Aspirate and wash 3 times.
5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C.
6. Aspirate and wash 5 times.
7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C.
8. Add 50ul Stop Solution. Read at 450nm immediately.