Comparison

Follicle Stimulating Hormone (FSH) BioAssay™ ELISA Kit (Rat)

Item no. USB-355751
Manufacturer United States Biological
Amount 48 tests
Quantity options 48 tests 96 tests
Category
Type Elisa-Kit
Applications ELISA
Specific against other
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Shipping Condition Cool pack
Available
Manufacturer - Category
Kits and Assays / Kits-ELISA (Hormones & Steroids), BioAssay™
Shipping Temperature
Blue Ice
Storage Conditions
4°C/-20°C
EU Commodity Code
38220000
Description
The Follicle Stimulating Hormone (FSH) BioAssay™ ELISA Kit is a quantitative competitive assay for the detection of FSH in rat serum, plasma, tissue homogenates and other biological fluids.

Detection Range:
2.344-150mIU/ml

Sensitivity:
<1.406mIU/ml

Precision:
Intra-assay CV: <8%
Inter-assay CV: <10%

Kit Components:
*355751A: Microtiter Strips, 1x96 wells (8x12 wells).
*355751B: Standard, 2x1 vial
355751C: Sample/Standard Dilution Buffer, 1x20ml
355751D: Antibody (Biotin) (Concentrated), 1x60ul
355751E: Antibody Dilution Buffer, 1x10ml
355751F: Streptavidin (HRP) (SABC), 1x120ul
355751G: SABC Dilution Buffer, 1x10ml
355751H: TMB Substrate, 1x10ml
355751J: Stop Solution, 1x10ml
355751K: Wash Buffer, 25X, 1x30ml

Storage and Stability:
Store unopened *355751A at 4ºC; store at -20ºC once opened. Store unopened *355751B at 4°C; once reconstituted, store at 4°C for up to 12 hours or at -20°C for up to 48 hours. Store other components at 4°C. Kit is stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.

Assay Principle:
This ELISA kit employs the quantitative competitive enzyme-linked immunoassay technique, utilizing a microtiter plate provided in this kit has been pre-coated with FSH. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to FSH. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FSH, Biotin-conjugated antibody and enzyme-conjugated Streptavidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FSH in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Assay Summary:
1. Wash plate 2 times before adding standards and samples to wells.
2. Add 50ul standard or sample to each well.
3. Immediately add 50ul Antibody-Biotin working solution to each well and incubate for 45 minutes at 37°C
4. Aspirate and wash 3 times.
5. Add 100ul SABC working solution to each well. Incubate for 30 minutes at 37°C
6. Aspirate and wash 5 times.
7. Add 90ul TMB substrate. Incubate 15-20 minutes at 37°C
8. Add 50ul Stop Solution. Read at 450nm immediately.
9. Calculate results.
Shelf Life
6 months

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 48 tests
Available: In stock
available

Compare

Add to wishlist

Get an offer

Request delivery time

Ask a technical question

Submit a bulk request

Questions about this Product?
 
Close