DOPA Decarboxylase/DDC ELISA Kit, human

This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of human DDC. The Human DOPA Decarboxylase/DDC ELISA is for research use only. Not for diagnostic or therapeutic procedures. DOPA decarboxylase (DDC), also known as aromatic-L-amino acid decarboxylase, is a 54 kDa lyase enzyme. It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3, 4 -dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan (5-HTP) to dopamine and serotonin, respectively. In normal dopamine and serotonin neurotransmitter synthesis, DDC is not the rate-limiting step in either reaction. However, DDC becomes the rate-limiting step of dopamine synthesis in patients treated with L-DOPA (such as in Parkinson's Disease), and the rate-limiting step of serotonin synthesis in people treated with 5-HTP (such as in mild depression or dysthymia). DDC is inhibited by Carbidopa outside of the blood brain barrier to inhibit the premature conversion of L-DOPA to dopamine in the treatment of Parkinson's. In humans, DDC is also the rate-limiting enzyme in the formation of trace amine neurotransmitters. Deficiency of DDC is associated with various symptoms as severe developmental delay, oculogyric crises and autonomic dysfunction.

Sandwich ELISA

serum, plasma, cell culture supernates

4C (unopened)
standard stored at -20C, others stored at 4C (opened)

1.02 pg/ml

81 % - 103 %

n.d. - 18334.5 pg/ml

2.0 % - 3.0 %