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AFP ELISA Kit (Human)

AFP ELISA Kit (Human)

Item no.

OKBB00766

Manufacturer

AVIVA Systems Biology

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Human (Homo sapiens)

Conjugate/Tag

Biotin

Sensitivity

<10 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Afp,Alpha fetoglobulin,Alpha-fetoglobulin,Alpha fetoprotein,Alpha-fetoprotein,Alpha-1-fetoprotein,FETA,HPAFP

Shipping Condition

Cool pack

Available

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits

Shipping Temperature

Wet Ice

Gene symbol

AFP

Gene Fullname

alpha fetoprotein

Reconstitution and storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

Alpha-fetoprotein (AFP, Î±-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown; however, in rodents it binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. As human AFP does not bind estrogen, its function in humans is less clear.

Nucleotide accession_num

NM_001134.2

Protein accession_num

NP_001125.1

Protein name

Alpha-fetoprotein

Immunogen

Expression system for standard: NS0; Immunogen sequence: R19-V609

Manufacturer - Specificity

Natural and recombinant human AFP

Kit duration

~ 3 Hours

Kit principle

Aviva's human AFP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for AFP has been precoated onto 96-well plates. Standards (Expression system for standard: NS0, Immunogen sequence: R19-V609) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for AFP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human AFP amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, cell lysates, serum and plasma (heparin, EDTA)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKBB00766.pdf

OKBB00766-datasheet.pdf

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Amount: 96 wells/kit, with removable strips.

available

Delivery expected until 1/8/2026

Express Delivery: Save 1 days

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