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CFH ELISA Kit (Human)

CFH ELISA Kit (Human)

Item no.

OKBB00960

Manufacturer

AVIVA Systems Biology

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Human (Homo sapiens)

Conjugate/Tag

Biotin

Sensitivity

<50 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

adrenomedullin binding protein,age related maculopathy susceptibility 1,AHUS 1,AHUS1,AMBP 1,AMBP1,ARMD 4,ARMD4,ARMS 1,ARMS1,beta 1 H globulin,beta 1H,beta1H,CFAH_HUMAN,CFH,CFHL 3,CFHL3,Complement factor H,complement factor H,isoform b,Factor H,factor H like 1,FH,FHL 1,FHL1,H factor 1 (complement),H factor 1,H factor 2 (complement),HF 1,HF 2,HF,HF1,HF2,HUS,MGC88246

Shipping Condition

Cool pack

Available

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits

Shipping Temperature

Wet Ice

Gene symbol

CFH

Gene Fullname

complement factor H

Reconstitution and storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

Complement factor H (CFH), originally known as beta-1H globulin, is a serum glycoprotein that regulates the function of the alternativecomplement pathway in fluid phase and on cellular surfaces. It binds to C3b, accelerates the decay of the alternative pathway convertase C3bBb, and also acts as a cofactor for complement factor I, another C3b inhibitor. The CFH gene is located on chromosome 1q32-q32.1 within a cluster of genes encoding the regulatory complement components of the activation of C3 (RCA for 'regulators of complement activation'). This gene cluster includes decay-accelerating factor (DAF), C4-binding protein (C4BPA and C4BPB), and the factor H-related genes CFHR1, CFHR2, CFHR3, CFHR4, and CFHR5, among others. The gene family has arisen by multiple duplication events.

Nucleotide accession_num

NM_000186.3

Protein accession_num

NP_000177.2

Protein name

Complement factor H

Immunogen

Expression system for standard: NS0; Immunogen sequence: S860-R1231

Manufacturer - Specificity

Natural and recombinant human CFH

Kit duration

~ 3 Hours

Kit principle

Aviva's human CFH ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CFH has been precoated onto 96-well plates. Standards (NS0, S860-R1231) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CFH is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human CFH amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, serum, plasma (heparin, EDTA) and urine

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKBB00960.pdf

OKBB00960-datasheet.pdf

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Amount: 96 wells/kit, with removable strips.

available

Delivery expected until 1/8/2026

Express Delivery: Save 8 days

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