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COL1A1 ELISA Kit (Human)

COL1A1 ELISA Kit (Human)

Item no.

OKBB01184

Manufacturer

AVIVA Systems Biology

Amount

96 wells/kit, with removable strips.

Category

Type

Elisa-Kit

Applications

ELISA

Specific against

Human (Homo sapiens)

Conjugate/Tag

Biotin

Sensitivity

<10 pg/ml

ECLASS 10.1

32160605

ECLASS 11.0

32160605

UNSPSC

41116126

Alias

Collagen alpha-1(I) chain; Alpha-1 type I collagen; COL1A1

Shipping Condition

Cool pack

Available

Manufacturer - Type

ELISA Kit

Manufacturer - Category

Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits

Shipping Temperature

Wet Ice

Gene symbol

COL1A1

Gene Fullname

collagen type I alpha 1 chain

Reconstitution and storage

Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Description of target

Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. And this gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Nucleotide accession_num

NM_000088.3

Protein accession_num

NP_000079.2

Protein name

Collagen alpha-1(I) chain

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q23-K277 + G1094-L1464

Manufacturer - Specificity

Natural and recombinant human COL1A1

Kit duration

~ 3 Hours

Kit principle

Aviva's human COL1A1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for COL1A1 has been precoated onto 96-well plates. Standards (Expression system for standard: CHO, Immunogen sequence: Q23-K277 + G1094-L1464) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for COL1A1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human COL1A1 amount of sample captured in plate.

Kit reproducibility

	Intra-Assay Precision			Inter-Assay Precision
Sample
Sample Type	cell culture supernates, serum and plasma (heparin, EDTA, citrate)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKBB01184.pdf

OKBB01184-datasheet.pdf

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Amount: 96 wells/kit, with removable strips.

available

Delivery expected until 12/25/2025

Express Delivery: Save 2 days

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