Comparison

CD55 ELISA Kit (Human)

Item no. OKBB01325
Manufacturer AVIVA Systems Biology
Amount 96 wells/kit, with removable strips.
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens)
Conjugate/Tag Biotin
Sensitivity <50 pg/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Complement decay-accelerating factor; CD55; CR; DAF
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/Kit, Root Catalog/Products/ELISA Kits
Shipping Temperature
Wet Ice
Gene symbol
CD55
Gene Fullname
CD55 molecule (Cromer blood group)
Reconstitution and storage
Store at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Description of target
Complement decay-accelerating factor, also known as CD55 or DAF, is a protein that, in humans, is encoded by the CD55 gene. It is mapped to 1q32.2. This gene encodes a glycoprotein involved in the regulation of the complement cascade. Binding of the encoded protein to complement proteins accelerates their decay, thereby disrupting the cascade and preventing damage to host cells. Antigens present on this protein constitute the Cromer blood group system (CROM). Alternative splicing results in multiple transcript variants. The predominant transcript variant encodes a membrane-bound protein, but alternatively spliced transcripts may produce soluble proteins.
Nucleotide accession_num
NM_000574.4
Protein accession_num
NP_000565.1
Protein name
Complement decay-accelerating factor
Immunogen
Expression system for standard: NS0; Immunogen sequence: D35-S353
Manufacturer - Specificity
Natural and recombinant human CD55
Kit duration
~ 3 Hours
Kit principle
Aviva's human CD55 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD55 has been precoated onto 96-well plates. Standards(Expression system for standard: NS0, Immunogen sequence: D35-S353) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD55 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Fibronectin amount of sample captured in plate.
Kit reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample
Sample Type
cell culture supernates, serum and plasma (heparin, EDTA)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 wells/kit, with removable strips.
Available: In stock
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