IFI27 ELISA Kit (Human)

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IFI27

Store as indicated in product manual.

NM_001130080.2

Interferon alpha-inducible protein 27, mitochondrial

~ 3.5 Hours

Aviva Systems Biology IFI27 ELISA Kit (Human) (OKCA00702) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IFI27 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IFI27 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IFI27 captured in the well.

Intra-assay Precision: 3 samples with known low, middle and high level IFI27 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level IFI27 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%