C8G Chemi-Luminescent ELISA Kit (Human)

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

C8G

2°C to 8°C|-20°C

NM_000606

Complement component C8 gamma chain

Component: Amount
C8G Microplate: 96 Wells (12 x 8 Well strips)
C8G Lyophilized Standard: 2
100X C8G Biotinylated Detector Anitbody: 1 x 120 uL
100X Avidin-HRP Conjugate: 1 x 120 uL
Standard Diluent: 1 x 20 mL
Detector Antibody Diluent: 1 x 12 mL
Conjugate Diluent: 1 x 12 mL
30X Wash Buffer: 1 x 20 mL
100X Luminol Substrate: 1 x 2 mL
Substrate Diluent: 1 x 20 mL

2h, 40min

The microplate provided in this kit has been pre-coated with an antibody specific to Complement Component 8g (C8g). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Complement Component 8g (C8g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Complement Component 8g (C8g) level in the sample or standard.

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Component 8g (C8g) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Component 8g (C8g) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%