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CHRNA1 Chemi-Luminescent ELISA Kit (Human)

CHRNA1 Chemi-Luminescent ELISA Kit (Human)

Item no.	OKCD03375
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Type	Elisa-Kit
Applications	CLIA, IA
Specific against	Human (Homo sapiens)
Sensitivity	0.78 ng/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	acetylcholine receptor subunit alpha;acetylcholine receptor,nicotinic,alpha 1 (muscle);ACHRA;ACHRD;cholinergic receptor,nicotinic alpha 1;cholinergic receptor,nicotinic,alpha 1 (muscle);cholinergic receptor,nicotinic,alpha polypeptide 1 (muscle);CHRNA;CMS1A;CMS1B;CMS2A;FCCMS;muscle nicotinic acetylcholine receptor;nicotinic acetylcholine receptor alpha subunit;nicotinic cholinergic receptor alpha 1;SCCMS.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	CHRNA1
Gene Fullname	cholinergic receptor nicotinic alpha 1 subunit
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Nucleotide accession_num	NM_000079
Protein accession_num	NP_000070
Protein name	Acetylcholine receptor subunit alpha
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount CHRNA1 Microplate: 96 Wells (12 x 8 Well strips) CHRNA1 Lyophilized Standard: 2 100X CHRNA1 Biotinylated Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL 100X Luminol Substrate: 1 x 2 mL Substrate Diluent: 1 x 20 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) level in the sample or standard.
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Alpha 1 (CHRNa1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Tissue homogenates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD03375.pdf

OKCD03375-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 12/11/2025

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