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CP Chemi-Luminescent ELISA Kit (Human)

CP Chemi-Luminescent ELISA Kit (Human)

Item no.	OKCD03395
Manufacturer	AVIVA Systems Biology
Amount	96 Wells
Category	Neuroscience Neural Signaling ELISA ELISA & Immunoassays ELISA Kits Immunoassay CLIA
Type	Elisa-Kit
Applications	CLIA, IA
Specific against	Human (Homo sapiens)
Sensitivity	1.37 pg/mL
ECLASS 10.1	32160605
ECLASS 11.0	32160605
UNSPSC	41116126
Alias	ceruloplasmin;ceruloplasmin (ferroxidase);CP-2;Ferroxidase.
Shipping Condition	Cool pack
Available
Manufacturer - Type	ELISA Kit
Manufacturer - Category	Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature	Wet Ice
Gene symbol	CP
Gene Fullname	ceruloplasmin
Reconstitution and storage	2°C to 8°C\|-20°C
Description of target	Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense (By similarity).
Nucleotide accession_num	NM_000096
Protein accession_num	NP_000087
Protein name	Ceruloplasmin
Assay info	Assay Methodology: Quantitative Sandwich ELISA
Kit Components	Component: Amount CP Microplate: 96 Wells (12 x 8 Well strips) CP Lyophilized Standard: 2 100X CP Biotinylated Detector Anitbody: 1 x 120 uL 100X Avidin-HRP Conjugate: 1 x 120 uL Standard Diluent: 1 x 20 mL Detector Antibody Diluent: 1 x 12 mL Conjugate Diluent: 1 x 12 mL 30X Wash Buffer: 1 x 20 mL 100X Luminol Substrate: 1 x 2 mL Substrate Diluent: 1 x 20 mL
Kit detection	Chemiluminescent
Kit duration	2h, 40min
Kit linearity	Sample - 1:2 - 1:4 - 1:8 - 1:16 Serum (n=5) - 80-90% - 82-89% - 81-96% - 81-98% EDTA Plasma (n=5) - 92-99% - 99-105% - 88-95% - 86-94% Heparin Plasma (n=5) - 83-90% - 79-89% - 82-96% - 93-103%
Kit principle	The microplate provided in this kit has been pre-coated with an antibody specific to Ceruloplasmin (CP). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ceruloplasmin (CP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ceruloplasmin (CP) level in the sample or standard.
Kit recovery	Matrix - Recovery range (%) - Average(%) Serum (n=5) - 98-105 - 102 EDTA Plasma (n=5) - 88-96 - 92 Heparin Plasma (n=5) - 78-104 - 83
Kit reproducibility	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ceruloplasmin (CP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ceruloplasmin (CP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Sample Type	Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

OKCD03395.pdf

OKCD03395-datasheet.pdf

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Amount: 96 Wells

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Delivery expected until 12/11/2025

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