Comparison

LCP1 ELISA Kit (Human)

Item no. OKDD00372
Manufacturer AVIVA Systems Biology
Amount 96 tests
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens)
Sensitivity < 0.054 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias bA139H14.1 (lymphocyte cytosolic protein 1 (L-plastin));CP64;epididymis secretory protein Li 37;HEL-S-37;LC64P;LCP-1;LPL;L-PLASTIN;L-plastin (Lymphocyte cytosolic protein 1) (LCP-1) (LC64P);Lymphocyte cytosolic protein 1;Lymphocyte cytosolic protein-1 (plasmin);plastin-2;PLS2.
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Molecular Weight
70 kDa
Gene symbol
LCP1
Gene Fullname
lymphocyte cytosolic protein 1
Protein size
627
Reconstitution and storage
Store as indicated in product manual.
Description of target
Actin-binding protein (PubMed:16636079, PubMed:17294403, PubMed:28493397). Plays a role in the activation of T-cells in response to costimulation through TCR/CD3 and CD2 or CD28 (PubMed:17294403). Modulates the cell surface expression of IL2RA/CD25 and CD69 (PubMed:17294403).
Nucleotide accession_num
NM_002298.4
Protein accession_num
NP_002289.2
Protein name
Plastin-2
Manufacturer - Specificity
This assay has high sensitivity and excellent specificity for detection of LCP1.No significant cross-reactivity or interference between LCP1 and analogues was observed.
Assay info
Quantitative Sandwich ELISA
Kit duration
~4 Hours
Kit principle
Aviva Systems Biology LCP1 ELISA Kit (Human) (OKDD00372) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for LCP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LCP1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample LCP1 captured in well.
Kit reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LCP1 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LCP1 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 tests
Available: In stock
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