MECP2 ELISA Kit (Human)

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52 kDa

methyl-CpG binding protein 2

Store as indicated in product manual.

NM_001110792.1

Methyl-CpG-binding protein 2

Quantitative Sandwich ELISA

Aviva Systems Biology MECP2 ELISA Kit (Human) (OKDD00389) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for MECP2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MECP2 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MECP2 captured in well.

Tissue homogenates, cell lysates, other biological fluids.