Comparison

NPTX1 ELISA Kit (Human)

Item no. OKDD00433
Manufacturer AVIVA Systems Biology
Amount 96 tests
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens)
Sensitivity < 0.049 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias neuronal pentraxin I;neuronal pentraxin-1;NP1;NP-I.
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Molecular Weight
47 kDa
Gene symbol
NPTX1
Gene Fullname
neuronal pentraxin 1
Protein size
432
Reconstitution and storage
Store as indicated in product manual.
Description of target
May be involved in mediating uptake of synaptic material during synapse remodeling or in mediating the synaptic clustering of AMPA glutamate receptors at a subset of excitatory synapses.
Nucleotide accession_num
NM_002522.3
Protein accession_num
NP_002513.2
Protein name
Neuronal pentraxin-1
Manufacturer - Specificity
This assay has high sensitivity and excellent specificity for detection of NPTX1.No significant cross-reactivity or interference between NPTX1 and analogues was observed.
Assay info
Quantitative Sandwich ELISA
Kit duration
~4 Hours
Kit principle
Aviva Systems Biology NPTX1 ELISA Kit (Human) (OKDD00433) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for NPTX1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPTX1 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample NPTX1 captured in well.
Kit reproducibility

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NPTX1 were tested 20 times on one plate, respectively.

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NPTX1 were tested on 3 different plates, 8 replicates in each plate.

Intra-Assay: CV<10%

Inter-Assay: CV<12%

Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 tests
Available: In stock
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