NT5E ELISA Kit (Human)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

63 kDa

5'-nucleotidase ecto

Store as indicated in product manual.

NM_001204813

P21589

Assay Methodology: Quantitative Sandwich ELISA

Aviva Systems Biology NT5E ELISA Kit (Human) (OKEH00185) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NT5E has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NT5E is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NT5E captured in well.

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids