GUSB ELISA Kit (Human)

ELISA Kit

Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit

75 kDa

glucuronidase beta

Store as indicated in product manual.

NM_000181.3

P08236

Assay Methodology: Quantitative Competitive ELISA

Aviva Systems Biology GUSB ELISA Kit (Human) (OKEH00293) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human GUSB antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated GUSB and incubated. The GUSB found in the sample or standards competes with the biotinylated GUSB for limited binding sites on the immobilized anti-human GUSB antibody. Excess unbound biotinylated GUSB and sample or standard GUSB is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated GUSB captured in the well and inversely proportional to the amount of GUSB which was contained in the sample or standard.

Mean Intra-assay CV%: < 2.8% (n = 20)
Mean Inter-assay CV%: < 7.3% (n = 20)