Comparison

PIK3CD ELISA Kit (Human)

Item no. OKEH02353
Manufacturer AVIVA Systems Biology
Amount 96 tests
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens)
Sensitivity 0.089 ng/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias APDS;IMD14;IMD14A;IMD14B;p110D;P110DELTA;Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta;phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform;phosphatidylinositol-4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta;phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta short variant 20;phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta short variant 8;phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta short variant 8/20;phosphoinositide-3-kinase C;phosphoinositide-3-kinase,catalytic,delta polypeptide variant p37delta;PI3K;PI3Kdelta;PI3-kinase p110 subunit delta;ptdIns-3-kinase subunit p110-delta;ROCHIS.
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA Kit
Manufacturer - Applications
Enzyme-linked Immunosorbent assay-Sandwich
Manufacturer - Category
Root Catalog/Products/ELISA Kits, Root Catalog/Products/Kit
Shipping Temperature
Wet Ice
Molecular Weight
119 kDa
Gene symbol
PIK3CD
Gene Fullname
phosphatidylinositol-4, 5-bisphosphate 3-kinase catalytic subunit delta
Protein size
1044
Reconstitution and storage
Store as indicated in product manual.
Description of target
Phosphatidylinositol 4, 5-bisphosphate 3-kinase catalytic subunit delta isoform
Nucleotide accession_num
NM_005026.3
Protein accession_num
NP_005017.3
Protein name
O00329
Manufacturer - Specificity
Natural and recombinant Human Phosphatidylinositol 4, 5-bisphosphate 3-kinase catalytic subunit delta isoform
Assay info
Assay Methodology: Quantitative Sandwich ELISA
Kit duration
~ 3 Hours
Kit principle
Aviva Systems Biology PIK3CD ELISA Kit (Human) (OKEH02353) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PIK3CD has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PIK3CD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PIK3CD captured in well.
Kit recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99%
Kit reproducibility
Mean Intra-assay CV%: < 5.4% (n = 20)
Mean Inter-assay CV%: < 9.7% (n = 20)
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 tests
Available: In stock
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