Carbonhydrate antigen 19-9 (CA19-9) BioAssay™ ELISA Kit (Human)

Kits and Assays / Kits-ELISA (Tumor Markers), BioAssay™

4°C/-20°C

Sample Type:
Serum, plasma

Intended Use:
For the quantitative determination of human carbonhydrate antigen 19-9 (CA19-9) concentrations in serum, plasma.

Sensitivity:
2.5U/ml

Range:
10-150U/ml

Test Principle:
This BioAssay™ employs the quantitative sandwich enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antibody specific for CA19-9. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for CA19-9 and incubated. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CA19-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Specificity:
This assay has high sensitivity and excellent specificity for detection of human CA19-9. No significant cross-reactivity or interference between human CA19-9 and analogues was observed.

Kit Components:
Assay plate, 1x96 wells
Standard, 4x0.3ml
HRP-conjugate, 1x6ml
Wash Buffer (20X), 1x15ml
Substrate A, 1x7ml
Substrate B, 1x7ml
Stop Solution, 1x7ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.