Zona pellucida Antibody (aZP) BioAssay™ ELISA Kit (Human)

Kits and Assays / Kits-ELISA, BioAssay™

4°C/-20°C

For the qualitative determination of human anti-zona pellucida antibody (aZP) concentrations in serum.

Specificity:
This assay has high sensitivity and excellent specificity for detection of human anti-zona pellucida antibody (aZP). No significant cross-reactivity or interference between human anti-zona pellucida antibody (aZP) and analogues was observed.

Test Principle:
This BioAssay™ employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human anti-zona pellucida antibody (aZP) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Sample Volume:
50-100ul

Detection Wavelength:
450nm

Precision:
Intra-assay: CV% <15%
Inter-assay: CV% <15%

Kit Components:
Coated assay plate, 1x96 wells
Negative Control, 1x0.8ml
Positive Control, 1x0.8ml
HRP-conjugate, 1x10ml
Sample Diluent, 1x20ml
Wash Buffer (25X), 1x20ml
Substrate A, 1x5ml
Substrate B, 1x5ml
Stop Solution, 1x5ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.