NF-κB p65 Antibody

WB, ELISA. Not yet tested in other applications. Determining optimal working dilutions by titration test.

Store at 4C, for long term storage, store at -20C

Purified recombinant fragment of human NF-kappaB p65 expressed in E. Coli.

Transcription factors of the nuclear factor kappa B (NF-kappaB)/Rel family is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. There are five family members in mammals. RelA (p65), c-Rel, RelB, NF-kappaB1 (p105/p50) and NF-kappaB2 (p100/p52). The most studied NF-kappaB complex consists of the p50 and p65 subunits, both containing a 300 amino acid region with homology to the Rel proto-oncogene product. The p50 subunit binds DNA, whereas the p65 subunit is responsible for the interaction of NF-kappaB with its inhibitor, IkappaB. In most cell types, the p50/p65 heterodimer is located within the cytoplasm complexed to IkappaB. This complex prevents nuclear translocation and activity of NF-kappaB. In response to stimuli such as cytokines, LPS, and viral infections, IkappaB is phosphorylated at critical residues. This phosphorylation induces dissociation of the IkappaB/NF-kappaB complex, allowing the free heterodimeric NF-kappaB to form a heterotetramer that translocates to the nucleus. In the nucleus, it binds to the kappaB site within promoters and enhancers and functions as a transcriptional activator.

Ascitic fluid containing 0.03% sodium azide.