TNF-α Antibody

This antibody is also available with the following conjugates:
AF350, AF405L, AF405M, AF405S, AF488, AF514, AF532, AF546, AF555, AF568, AF594, AF610, AF635, AF647, AF680, AF700, AF750, AF790, APC, AP, Biotin, Cy3, Cy5.5, Cy5, Cy7, FITC, HRP, PE, Magnetic beads (1 um, 2.8 um, 3 um, 4.5 um, 5 um, 10 um, 15 um, 20 um, 30 um, or different size option)
Please contact sales@hoelzel.de for pricing and availability.

26kDa

Affinity purification

TNF-alpha, the prototypical member of the TNF protein superfamily, is a homotrimeric type-II membrane protein (1, 2). Membrane-bound TNF-alpha is cleaved by the metalloprotease TACE/ADAM17 to generate a soluble homotrimer (2). Both membrane and soluble forms of TNF-alpha are biologically active. TNF-alpha is produced by a variety of immune cells including T cells, B cells, NK cells, and macrophages (1). Cellular response to TNF-alpha is mediated through interaction with receptors TNF-R1 and TNF-R2 and results in activation of pathways that favor both cell survival and apoptosis depending on the cell type and biological context. Activation of kinase pathways (including JNK, Erk (p44/42), p38 MAPK, and NF-kappaB) promotes the survival of cells, while TNF-alpha-mediated activation of caspase-8 leads to programmed cell death (1, 2). TNF-alpha plays a key regulatory role in inflammation and host defense against bacterial infection, notably Mycobacterium tuberculosis (3). The role of TNF-alpha in autoimmunity is underscored by blocking TNF-alpha action to treat rheumatoid arthritis and Crohn’s disease (1, 2, 4).