miRNA Isolation Kit (Tri-reagent + RNA spin column)

centrifugation and vaccum
Favorgen miRNA Isolation Kit is designed for purification of microRNAs (miRNAs) and other small cellular RNAs from tissue
samples or cultured cells. Purification of miRNAs allows research into biological significant pathways for regulation of gene
regulation.
The standard protocols for isolating total RNA and mRNA are not optimized for isolation of small RNA molecules and result in the
loss of substantial amounts of miRNAs and other small RNA. In addition, removal of the predominant larger RNAs is required for
accurate analysis of miRNA expression by qPCR or microarray analysis.
This kit is specifically designed for purification of small RNA with minimal contamination from large RNA molecules or genomic
DNA. The method employs a spin column with a silica-based fiber matrix that binds RNA in the presence of a chaotropic salt.
The method is based on the selective binding of RNA molecules of different sizes to the silica-based fiber matrix when different
ethanol concentrations are present in the solvent.

~ 30 minutes

up to 1 x10 cultured cells
up to 100 mg tissue