Presenilin 1 Elisa Kit (human, mouse, rat, canine)

Sodium Azide (0.1%)

Presenilins (Presenilin 1 and Presenilin 2) are multipass-integral membrane proteins thought to function as the catalytic subunit of the gamma-secretase complex, a protease complex that catalyzes the cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Presenilins also cleave E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin and negatively regulating Wnt signaling.

The gamma-secretase complex is composed of presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2, and additional proteins. These proteins are sufficient for catalytic activity. Other components associated with the gamma-secretase complex include SLC25A64, SLC5A7, and PHB. Presenilin 1 is located in the endoplasmic reticulum membrane, Golgi membrane and cell surface membrane. Presenilin-1 is expressed in a wide range of tissues that include brain, liver, spleen and lymph nodes.

Icon Image: Solution structure of Presenilin 1 of amino acids 292-497.

Ankarcrona, M., & Hultenby, K. (2002). Presenilin-1 is located in rat mitochondria. Biochemical and Biophysical Research Communications, 295(3), 766-770. Crump, C. J., Fish, B. A., Castro, S. V., Chau, D.-M., Gertsik, N., Ahn, K., Stiff, C., et al. (2011). Piperidine acetic acid based gamma-secretase modulators directly bind to Presenilin-1. ACS chemical neuroscience, 2(12), 705-710.

De Gasperi, R., Sosa, M. A. G., Dracheva, S., & Elder, G. A. (2010). Presenilin-1 regulates induction of hypoxia inducible factor-1alpha: altered activation by a mutation associated with familial Alzheimer’s disease. Molecular neurodegeneration, 5(1), 38. BioMed Central Ltd.

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Kandimalla, R. J., Wani, W. Y., Bk, B., & Gill, K. D. (2012). siRNA against presenilin 1 (PS1) down regulates amyloid beta 42 production in IMR-32 cells. Journal of biomedical science, 19(1), 2.

Koizumi, K., Nakajima, M., Yuasa, S., Saga, Y., Sakai, T., Kuriyama, T., Shirasawa, T., et al. (2001). The role of presenilin 1 during somite segmentation. Development Cambridge England, 128(8), 1391-1402.

Refolo, L. M., Eckman, C., Sanders, S., Yager, D., Perez-tur, J., Younkin, S., et al. (1998). Presenilin 1 Mutations. Human Molecular Genetics, 42(43), 256-258.