NF-kappa B, NF-{kappa}B, NF-kB, Nuclear Factor-Kappa B, Activation Assay Kit (Part No. NFKB-1)

Rapid assay to detect and quantitate NF-kappa B activation by isolating nuclear and cytoplasmic fractions and detection of NF-KB transcription factor subunit (i.e. p65 and p50) enrichment in the nuclear fraction by Western blot.
Nuclear and cytoplasmic fractions are isolated in less than 1 hour using a microcentrifuge.
Data from this NF-KB Activation Assay Kit are higher resolved, easier to quantitate and more convincing than fluorescence and luminescence assays, including ELISA.
The NF-KB Activation Assay Kit is applicable in virtually all mammalian cells.
For 50 to 150 NF-kappa B activation assays based on one assay per semi-confluent 10 cm cell culture dish or well of a 6-well dish, respectively.

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