Rabbit Amyloid-Beta (1 - 42) ELISA Assay Kit, 96T

Sodium Azide (0.1%)

The provided 96-well ELISA micro-strip plate is pre-coated with an affinity purified rabbit polyclonal antibody selective to the antigen. Upon addition of samples to the wells, the antigen binds to the antibody and becomes indirectly linked to the solid support. After washes of unbound materials, a second affinity purified polyclonal antibody conjugated with horse-radish peroxidase (HRP) is added, followed by a second incubation period. Unbound antibody is washed, and color reagents are added, which upon conversion by HRP, become blue colored. A stop solution is then added to block further reaction between HRP and the colorimetric substrates, causing the solution to become yellow. An absorbance multiplate reader is used to quantitate the colorimetric reaction at 450 nm.

These ELISA kits provide a standard peptide solution with known concentration to calibrate absorbance readings to concentration.

Amyloid-Beta (A&eta) is generated by sequential cleavage of the amyloid precursor protein (APP) by beta and gamma secretases. Inhibition of secretase activity has been proposed as a therapeutic strategy to limit amyloid-Beta (Abeta) accumulation. Gamma-secretase, which produces the C-terminal end of the Abeta peptide, cleaves within the transmembrane region of APP and generates amyloid-Beta (Abeta) isoforms of 36-43 amino acid residues in length. The dominant isoforms of amyloid-Beta are Abeta40 and Abeta42. Abeta40 is the most common amyloid-Beta (Abeta) isoform, whereas Abeta42 is the more fibrillogenic and the predominant amyloid-beta peptide associated Alzheimer’s disease. Antibodies employed in this Amyloid-Beta ELISA kit are selective for the Abeta42 isoform of amyloid-beta, with insignificant cross-reactivity to the Abeta40 isoform.