Highlights |
Available in 1 ml or 5 ml stock 100X water soluble phosphatse inhibitor cocktail solution. Add directly to cell lysates or culture medium. Broad spectrum, highly potent: Inhibits seryl, threonyl, tyrosyl, alkaline and Ca2+-phosphatases Contains activated activated sodium orthovanadate, sodium tartrate, sodium fluoride, beta-glycerophosphate. Ships at ambient temperature. Store at 4C short term (4 months) or -20C long term (2 years). |
References |
Arnold WN, Sakai KH, and Mann LC. Selective inactivation of an extra-cytoplasmic acid phosphatase of yeast-like cells of Sporothrix schenckii by sodium fluoride. J Gen Microbiol 133: 1503-1509, 1987.
Botana LM, Alfonso A, Botana MA, Vieytes MR, Louzao MC, and Cabado AG. Influence of protein kinase C, cAMP and phosphatase activity on histamine release produced by compound 48/80 and sodium fluoride on rat mast cells. Agents Actions 37: 1-7, 1992.
Gordon JA. Use of vanadate as protein-phosphotyrosine phosphatase inhibitor. Methods Enzymol 201: 477-482, 1991.
Guideri R, Cesari L, Burghesi R, and Medaglini E. [Behavior of calcemia, phosphoremia, calciuria, phosphaturia and alkaline phosphatase in normal subjects treated with sodium fluoride (NaF)]. Boll Soc Ital Biol Sper 42: 1612-1615, 1966.
Lange AJ, Arion WJ, Burchell A, and Burchell B. Aluminum ions are required for stabilization and inhibition of hepatic microsomal glucose-6-phosphatase by sodium fluoride. J Biol Chem 261: 101-107, 1986.
Li L, Eisen AZ, Sturman E, and Seltzer JL. Protein tyrosine phosphorylation in signalling pathways leading to the activation of gelatinase A: activation of gelatinase A by treatment with the protein tyrosine phosphatase inhibitor sodium orthovanadate. Biochim Biophys Acta 1405: 110-120, 1998.
Suketa Y, Kuwahara M, Sahashi K, Suzuki N, and Akiyama Y. A role of renal K+-stimulated phosphatase activity on elevation of urinary sodium excretion in fluoride-treated rats. Toxicol Appl Pharmacol 69: 225-233, 1983. |