FIVEphoton Fluorescent Membrane Potential Assay Kit - High Throughput Format

Manufacturer Fivephoton
Type Kit
Specific against other
Amount 1 kit
Item no. mpHTS-Kit-B
Eclass 6.1 32161000
Eclass 9.0 32161090
Fast response time of indicator dyes
No requirement for pre-assay soaking or washes
Ability to perform assays at room or physiological temperatures
Highly reproducible and reliable fluorescence data
Streamlined reagent preparation and system optimization
The bilipid membrane of a cell typically has a transmembrane potential of approximately -60 to -80 mV (negative inside) as a consequence of mostly Na+, K+, and Cl- ion concentrations gradients which are maintained by active ion transporters. Voltage sensitive probes (VSP) offer a convenient method of detecting the translocation of these ions across cellular membranes. The thiobarbituric and bis-barbituric acid oxonols, referred to as DiSBAC2 and DiBAC3 dyes respectively, are VSPs with distinct excitation and emission parameters. The FIVEphoton Biochemicals membrane potential assay kits utilize a dye formulation which is very similar to the most popular oxonol dyes for transmembrane potential measurement. Membrane potential dyes enter depolarized cells and associate with intracellular proteins or membranes, causing enhanced fluorescence and a spectral shift to red wavelengths. Increase in membrane depolarization leads to further influx of dye and thus an increase in fluorescence that can be measured by fluorescence spectroscopy and fluorescent plate readers.

The membrane potential assay dyes can be used to measure transmembrane potential in living cells as a stand alone reagent or in combination with other fluorescent indicators. The dye systems are recommended for use in fluorescent plate readers, fluorometers, and flow cytometers.
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Amount: 1 kit
Available: In stock
Listprice: €1,828.79
Price: €1,828.79

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